The Pichia pastoris dihydroxyacetone kinase is a PTS1‐containing, but cytosolic, protein that is essential for growth on methanol
نویسندگان
چکیده
منابع مشابه
The Pichia pastoris dihydroxyacetone kinase is a PTS1-containing, but cytosolic, protein that is essential for growth on methanol.
Dihydroxyacetone kinase (DAK) is essential for methanol assimilation in methylotrophic yeasts. We have cloned the DAK gene from Pichia pastoris by functional complementation of a mutant that was unable to grow on methanol. An open reading frame of 1824 bp was identified that encodes a 65.3 kDa protein with high homology to DAK from Saccharomyces cerevisiae. Although DAK from P. pastoris contain...
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ژورنال
عنوان ژورنال: Yeast
سال: 1998
ISSN: 0749-503X,1097-0061
DOI: 10.1002/(sici)1097-0061(19980615)14:8<759::aid-yea275>3.3.co;2-1